Journal: Scientific Reports

Article Title: Increase in fatty acids and flotillins upon resveratrol treatment of human breast cancer cells

doi: 10.1038/s41598-019-50416-5

Figure Lengend Snippet: Effect of resveratrol on the cell viability of MCF-7 and MDA-MB-231 cells. ( a ) 4T1, ( b ) MCF-7 and ( c ) MDA-MB-231 cells (2 × 10 5 ) were incubated in the presence of different concentrations (10 to 300 μM) of resveratrol, or 0.1% DMSO (control), for 24 h at 37 °C. They were then washed and maintained with MTT (0.5 mg/mL) for 3 h at 37 °C for formazan crystals formation and the associated optical density was analyzed at 570 and 650 nm. The results were expressed as mean ± SD, n = 3, *p < 0.05, **p < 0.01, ***p < 0.001 significantly different (p < 0.05) from the values corresponding to the control, using One-way ANOVA, followed by multiple comparison test Dunnett.

Article Snippet: The human mammary gland/breast epithelial cell lines MCF-7 and MDA-MB-231, and the mouse mammary gland/breast epithelial cell line 4T1 were obtained from American Type Culture Collection (ATCC® HTB-22TM, HTB-26TM and CLR-2539TM respectively).

Techniques: Incubation, Control, Comparison